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galactose inducible promoter gal1 10  (Vector Laboratories)


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    Structured Review

    Vector Laboratories galactose inducible promoter gal1 10
    Galactose Inducible Promoter Gal1 10, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/galactose+inducible+gal1+promoter/galactose/pmc12441929-203-29-26
    Average 96 stars, based on 668 article reviews
    galactose inducible promoter gal1 10 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.
    Article Snippet: .. RGG constructs with a coiled coil or FRB and FKBP tags were cloned downstream of a galactose-inducible GAL1 promoter and integrated into the yeast URA3 locus using the Yiplac211 integrating vector or LEU2 locus using the Yiplac128 integrating vector by linearizing plasmid with EcoRV just before transformation. ..

    Clone Assay:

    Article Title: Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.
    Article Snippet: .. RGG constructs with a coiled coil or FRB and FKBP tags were cloned downstream of a galactose-inducible GAL1 promoter and integrated into the yeast URA3 locus using the Yiplac211 integrating vector or LEU2 locus using the Yiplac128 integrating vector by linearizing plasmid with EcoRV just before transformation. ..

    Plasmid Preparation:

    Article Title: Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.
    Article Snippet: .. RGG constructs with a coiled coil or FRB and FKBP tags were cloned downstream of a galactose-inducible GAL1 promoter and integrated into the yeast URA3 locus using the Yiplac211 integrating vector or LEU2 locus using the Yiplac128 integrating vector by linearizing plasmid with EcoRV just before transformation. ..

    Article Title: Target of Rapamycin and LST8 Proteins Associate with Membranes from the Endoplasmic Reticulum in the Unicellular Green Alga Chlamydomonas reinhardtii
    Article Snippet: .. Cells expressing LST8 from the glucose-repressible and galactose-inducible GAL1 promoter were transformed empty vector (vector) or with plasmids expressing wild-type CrLST8 or D106G, T228A, or S230N CrLST8 mutants. ..

    Transformation Assay:

    Article Title: Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.
    Article Snippet: .. RGG constructs with a coiled coil or FRB and FKBP tags were cloned downstream of a galactose-inducible GAL1 promoter and integrated into the yeast URA3 locus using the Yiplac211 integrating vector or LEU2 locus using the Yiplac128 integrating vector by linearizing plasmid with EcoRV just before transformation. ..

    Article Title: Target of Rapamycin and LST8 Proteins Associate with Membranes from the Endoplasmic Reticulum in the Unicellular Green Alga Chlamydomonas reinhardtii
    Article Snippet: .. Cells expressing LST8 from the glucose-repressible and galactose-inducible GAL1 promoter were transformed empty vector (vector) or with plasmids expressing wild-type CrLST8 or D106G, T228A, or S230N CrLST8 mutants. ..

    Expressing:

    Article Title: Target of Rapamycin and LST8 Proteins Associate with Membranes from the Endoplasmic Reticulum in the Unicellular Green Alga Chlamydomonas reinhardtii
    Article Snippet: .. Cells expressing LST8 from the glucose-repressible and galactose-inducible GAL1 promoter were transformed empty vector (vector) or with plasmids expressing wild-type CrLST8 or D106G, T228A, or S230N CrLST8 mutants. ..



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    galactose inducible gal1 promoter  (Vector Laboratories)
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    Vector Laboratories galactose inducible gal1 promoter
    Figure 4. Leveraging induced dimerization to trigger synthetic condensate formation in living cells. (A) Scheme for encoding and expression of RGG polypeptides in cells, sensitive to small-molecule-induced dimerization and condensation. RGG-GFP-FKBP and RGG-FRB constructs are integrated into the yeast genome controlled by an inducible <t>GAL1</t> promoter. (B) Representative images for strains described in A, showing addition of 20 μM Rap triggers condensate formation within minutes in live cells. (C) Average number of droplets formed per yeast cell in the n = 105 cells. Shaded area, 95% CI. (D) Optical regulation of condensation in cells following illumination to photouncage dRap. (E) Average number of droplets formed per cell following 10 s of 405 nm laser illumination (n = 66 cells). Shaded area, 95% CI.
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    Image Search Results


    Figure 4. Leveraging induced dimerization to trigger synthetic condensate formation in living cells. (A) Scheme for encoding and expression of RGG polypeptides in cells, sensitive to small-molecule-induced dimerization and condensation. RGG-GFP-FKBP and RGG-FRB constructs are integrated into the yeast genome controlled by an inducible GAL1 promoter. (B) Representative images for strains described in A, showing addition of 20 μM Rap triggers condensate formation within minutes in live cells. (C) Average number of droplets formed per yeast cell in the n = 105 cells. Shaded area, 95% CI. (D) Optical regulation of condensation in cells following illumination to photouncage dRap. (E) Average number of droplets formed per cell following 10 s of 405 nm laser illumination (n = 66 cells). Shaded area, 95% CI.

    Journal: Biochemistry

    Article Title: Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.

    doi: 10.1021/acs.biochem.2c00250

    Figure Lengend Snippet: Figure 4. Leveraging induced dimerization to trigger synthetic condensate formation in living cells. (A) Scheme for encoding and expression of RGG polypeptides in cells, sensitive to small-molecule-induced dimerization and condensation. RGG-GFP-FKBP and RGG-FRB constructs are integrated into the yeast genome controlled by an inducible GAL1 promoter. (B) Representative images for strains described in A, showing addition of 20 μM Rap triggers condensate formation within minutes in live cells. (C) Average number of droplets formed per yeast cell in the n = 105 cells. Shaded area, 95% CI. (D) Optical regulation of condensation in cells following illumination to photouncage dRap. (E) Average number of droplets formed per cell following 10 s of 405 nm laser illumination (n = 66 cells). Shaded area, 95% CI.

    Article Snippet: RGG constructs with a coiled coil or FRB and FKBP tags were cloned downstream of a galactose-inducible GAL1 promoter and integrated into the yeast URA3 locus using the Yiplac211 integrating vector or LEU2 locus using the Yiplac128 integrating vector by linearizing plasmid with EcoRV just before transformation.

    Techniques: Expressing, Construct